Hybridoma cell antibody gene sequencing:
        Traditional hybridoma can propagate infinitely with high yield of monoclonal antibodies, but this way of preparing monoclonal antibodies will also meet typical issues like gene loss or mutation which cause degradation or even failure of the antibody’s specificity, it could further lead to uncontrollable quality problem of monoclonal antibody’s stability. Through recombining, sequencing and cloning the gene of the target antibody in the hybridoma cell, we can not only keep the high specificity of monoclonal antibody, but also manage to reduce the deviation between batches of antibody production which makes antibodies quality more consistant and controllable. Antibody’s gene sequence acquisition can also strengthen the protection of intellectual property rights. So to obtain hybridoma monoclonal antibody gene sequences is significantly meaningful for stably large-scale production of monoclonal antibodies. In order to better meet the needs of scientific research users, Goodhere adopts professional scheme (variable region sequencing, whole sequencing, as shown in figure) to provide the antibody gene sequencing services. Compared with the amino acid sequence of monoclonal antibody, gene sequencing can provide more accurate, reliable and economic rapid results. We could also provide customized services according to specific needs.

         Highly stable quality cell strain is the key to the in vitro large-scale preparation of consistant antibody. The main way is to integrate the antibody’s gene into the chromosome of the host cell so as to let it express the antibody stably. How to construct a stable high quality cell strain is essential. As a professional research and development company of antibody, goodhere owns a series of stable cell line building technology and rich experience in mammalian cell culture. We could also provide customized services according to specific needs.

         FortebioAntibody affinity determination Biofilm interference technique can detect the reaction occurred on the surface of biosensor through observing the interference spectrum displacement. As a beam of visible light sent out from the spectrometer to the optical films at the tips of the biosensor, there forms two reflection spectra and one interference spectrum. Any molecules association or dissociative will affect the thickness and density of the optical films, this can be observed by the real-time displacement value on the interference spectrum.
Otec platform can be used for rapid determination of antibody and antigen reaction, and obtaining accurate affinity constant of antigen and antibody, which is helpful for subsequent antigen screening
·The results are comparable with HPLC, and the precision is better than ELISA
·Multiple samples can be tested in a short time
·Direct detection, no need of labeling and other reagents
·Without tedious purification engineering, it is possible to directly determine crude samples such as culture supernatant
·Customized services can be provided to make special biosensors according to users' needs

         Otec platform is based on the principle of biofilm interference (BLI) technology to achieve the detection of molecular interaction, including quantitative detection and kinetic detection. Biofilm interference technique can detect the reaction occurred on the surface of biosensor through observing the interference spectrum displacement. As a beam of visible light sent out from the spectrometer to the optical films at the tips of the biosensor, there forms two reflection spectra and one interference spectrum. Any molecules association or dissociative will affect the thickness and density of the optical films, this can be observed by the real-time displacement value on the interference spectrum.
We can use Octec platform to screening paired antibody which are widely used in sandwich method.
·We can use Octec platform to screening paired antibody which are widely used in sandwich method.
·Results can be observed in details on the spectrum diagram, which help to intuitively screen the optimal pairing scheme.
·Direct determination of the form, no need of labeling and other reagents
·Customized services can be provided to make special biosensors according to users' needs

         Otec platform is based on the principle of biofilm interference (BLI) technology to achieve the detection of molecular interaction, including quantitative detection and kinetic detection. Biofilm interference technique can detect the reaction occurred on the surface of biosensor through observing the interference spectrum displacement. As a beam of visible light sent out from the spectrometer to the optical films at the tips of the biosensor, there forms two reflection spectra and one interference spectrum. Any molecules association or dissociative will affect the thickness and density of the optical films, this can be observed by the real-time displacement value on the interference spectrum.
Otec platform can detect the interaction of various other substances
· Polysaccharide/Polypeptide/Virus interactions
· Bacteria/DNA interactions
· RNA/Nano-particles interactions
· Customized services can be provided to make special biosensors according to users' needs

         Clients can provide antigen to us to develop the monoclonal antibody(mouse-source). We use special adjuvant formulations, that strengthens response ability of immune cells, shortening the time of immune response, and extend the duration of immunity, improve the antibody’s titer and affinity. We would provide two hybridoma cell lines from which produced 10 ml culture supernatant and 10 ml mice ascites, the whole preparation process need 2.5 to 4 months including stability test.

         Clients can provide antigen to us to develop the polyclonal antibody(rabbit-source). We use special adjuvant formulations, that strengthens response ability of immune cells, shortening the time of immune response, and extend the duration of immunity, improve the antibody’s titer and affinity. We would provide 1ml pre-immunization serum and 45ml polyclonal antibody serum, ELISA titer greater than 1:15000, and the required time is only 40-45 days.